The Comprehensive Effects of Rhizophagus intraradices and P on Root System Architecture and P Transportation in Citrus limon L.

Both arbuscular mycorrhizal fungi (AMF) and phosphorus (P) collectively influence the root system architecture (RSA), but whether combination of two affects RSA, particularly lateral formation, is unknown. In present study, a pot experiment was conducted to evaluate effects an fungus (Rhizophagus intraradices) on RSA lemon (Citrus limon L.) seedlings under 0 (P0) 50 mg/kg (P50) P levels. Moreover, carbohydrate content; acid phosphatase activity; expression transporter genes (PTs), (PAPs), lateral-root-related genes; were determined. Our results show that colonization dependency plants are significantly higher P0 than P50 conditions. AMF promoted plant growth performance lemon, irrespective substrate The parameters plants, including total length, projected area, surface average diameter, volume, second- third-order numbers, distinctly increased levels compared those non-AMF plants. Mycorrhizal treatment also induced (sucrose, glucose, fructose) contents, along with activity phosphatase. P-related genes, ClPAP1, ClPT1, ClPT3, ClPT5, ClPT7, as well (ClKRP6, ClPSK6, ClRSI-1), dramatically upregulated by inoculation, Principal component analysis showed ClKRP6 positively correlated traits development. study demonstrates mycorrhizas accelerate acquisition accumulation upregulating thereby improving RSA. Furthermore, had greater impact